rna probes การใช้

• The photoactivatable group provides nonspecific labeling of proteins, DNA and RNA probes or other molecules.
• RNA probes complementary to mRNA targets trigger chain reactions in which fluorophore-labeled scRNAs self-assemble into tethered fluorescent amplification polymers.
• By using RNA probes, Todd Oakley research group determined that one of the two cryptochromes, Aq-Cry2, was produced near the sponge s simple eye cells.
• The probe hybridizes to the target sequence at elevated temperature, and then the excess probe is washed away ( after prior hydrolysis using RNase in the case of unhybridized, excess RNA probe ).
• The recombinant DNA may also be detected using other methods such as nucleic acid hybridization with radioactive RNA probe, while cells that expressed the desired protein from the plasmid may also be detected using immunological methods.
• In situ hybridization uses synthetic RNA probes that attach ( hybridize ) selectively to complementary mRNA transcripts of DNA exons in the cytoplasm, to visualize genomic readout, that is, distinguish active gene expression, in terms of mRNA rather than protein.
• The key techniques currently in use include : in situ hybridization to mRNA with oligonucleotide and RNA probes ( both radio-labelled and hapten-labelled ); analysis with light and electron microscopes; whole mount in situ hybridization; double detection of RNAs and RNA plus protein; and fluorescent in situ hybridization to detect chromosomal sequences.